Characterization of Oxidative Enzyme Changes in Buffalograsses Challenged by <i>Blissus occiduus</i>
نویسندگان
چکیده
This research investigated the role of oxidative enzymes in the defense response of buffalograss,Buchloë dactyloides (Nuttall) Engelmann, toBlissus occiduusBarber. Changes in catalase and peroxidase activity were observed in both resistant and susceptible buffalograsses in response to chinch bug feeding. Susceptible plants were shown to have a lower level of catalase activity compared with their respective control plants. By contrast, catalase activities of resistant plants were similar between infested and control buffalograsses throughout the study. Resistant plants had higher levels of peroxidase activity comparedwith their control plants,whereas peroxidase activities for control and infested susceptible plants remained at similar levels or were slightly lower for infested plants. These Þndings suggest that chinchbug feeding leads to a loss in catalase activity in susceptible buffalograsses. In contrast, resistant buffalograsses may be able to tolerate chinch bug feeding by increasing their peroxidase activity. Polyphenol oxidase activities were similar between control and infested plants for the buffalograsses evaluated. Among the enzymes examined, no differences in isozyme proÞles for peroxidase and polyphenol oxidasewere detected between control and infested 378, NE91-118, Cody, and Tatanka plants. Gels stained for catalase identiÞed differences in the isozyme proÞles of infested and uninfested 378 plants; however, infested and control NE91-118, Tatanka, and Cody plants has similar isozyme proÞles. No differences in protein proÞles were observed between chinch buginfested 378, NE91-118, Cody, and Tatanka plants and their respective uninfested controls.
منابع مشابه
Characterization of oxidative enzyme changes in buffalograsses challenged by Blissus occiduus.
This research investigated the role of oxidative enzymes in the defense response of buffalograss, Buchloë dactyloides (Nuttall) Engelmann, to Blissus occiduus Barber. Changes in catalase and peroxidase activity were observed in both resistant and susceptible buffalograsses in response to chinch bug feeding. Susceptible plants were shown to have a lower level of catalase activity compared with t...
متن کاملPhysiological Responses of Resistant and Susceptible Buffalograsses to <i>Blissus Occiduus</i> (Hemiptera: Blissidae) Feeding
The impact of Blissus occiduus Barber feeding on resistant (ÔPrestigeÕ) and susceptible (Ô378Õ) buffalograsses,Buchloë dactyloides (Nuttall) Engelmann, was evaluated throughmeasurement of carbon exchange rate, light and carbon assimilation (A-Ci) curves, chlorophyll a ßuorescence, and nonstructural carbohydrates. No signiÞcant differences in carbon exchange rates were observed between infested ...
متن کاملPhysiological responses of resistant and susceptible buffalograsses to Blissus occiduus (Hemiptera: Blissidae) feeding.
The impact of Blissus occiduus Barber feeding on resistant ('Prestige') and susceptible ('378') buffalograsses, Buchloë dactyloides (Nuttall) Engelmann, was evaluated through measurement of carbon exchange rate, light and carbon assimilation (A-C(i)) curves, chlorophyll a fluorescence, and nonstructural carbohydrates. No significant differences in carbon exchange rates were observed between inf...
متن کاملEvaluation of Buffalograss Germplasm for Resistance to <i>Blissus occiduus </i> (Hemiptera: Lygaeidae)
Blissus occiduusBarber has emerged as an important insect pest of buffalograss,Buchloë dactyloides (Nuttall) Engelmann, in Nebraska. This research evaluated selected buffalograss germplasm for resistance to B. occiduus. Eleven buffalograss selections were screened for chinch bug resistance in three greenhouse studies and two Þeld evaluations. Based on chinch bug damage, NE91Ð118, ÔTatankaÕ, ÔBo...
متن کامل